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Medium optimization of protease production by Brevibacterium linens DSM 20158, using statistical approach
Shabbiri, Khadija; Adnan, Ahmad; Jamil, Sania; Ahmad, Waqar; Noor, Bushra; Rafique, H.M..
Afiliação
  • Shabbiri, Khadija; GC University Department of Chemistry.
  • Adnan, Ahmad; GC University Department of Chemistry.
  • Jamil, Sania; GC University Department of Chemistry.
  • Ahmad, Waqar; University of the Punjab National Centre of Excellence in Molecular Biology.
  • Noor, Bushra; GC University Department of Chemistry.
  • Rafique, H.M.; University of the Punjab Department of Physics.
Braz. J. Microbiol. ; 43(3)2012.
Article em En | VETINDEX | ID: vti-444956
Biblioteca responsável: BR68.1
ABSTRACT
Various cultivation parameters were optimized for the production of extra cellular protease by Brevibacterium linens DSM 20158 grown in solid state fermentation conditions using statistical approach. The cultivation variables were screened by the Plackett-Burman design and four significant variables (soybean meal, wheat bran, (NH4)2SO4 and inoculum size were further optimized via central composite design (CCD) using a response surface methodological approach. Using the optimal factors (soybean meal 12.0g, wheat bran 8.50g, (NH4)2SO4) 0.45g and inoculum size 3.50%), the rate of protease production was found to be twofold higher in the optimized medium as compared to the unoptimized reference medium.
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2012 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Base de dados: VETINDEX Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2012 Tipo de documento: Article