Active human Cytomegalovirus infection and Glycoprotein B genotypes in Brazilian pediatric renal or hematopoietic stem cell transplantation patients

de Campos Dieamant, Débora; Helena Alves Bonon, Sandra; Cury Prates, Liliane; Maria Santoro Belangelo, Vera; ldrinildrinildrinildrinildrinildrinildrini; Cecília Botelho Costa, Sandra

de Campos Dieamant, Débora; Helena Alves Bonon, Sandra; Cury Prates, Liliane; Maria Santoro Belangelo, Vera; ldrinildrinildrinildrinildrinildrinildrini; Cecília Botelho Costa, Sandra.

Afiliação

de Campos Dieamant, Débora; Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médica.
Helena Alves Bonon, Sandra; Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médica.
Cury Prates, Liliane; Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Nefrologia Pediátrica.
Maria Santoro Belangelo, Vera; Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Nefrologia Pediátrica.
ldrinildrinildrinildrinildrinildrinildrini; s.af
Cecília Botelho Costa, Sandra; Universidade Estadual de Campinas Faculdade de Ciências Médicas Departamento de Clínica Médica.

Braz. j. microbiol ; Braz. j. microbiol;41(1)Jan.-Mar. 2010.

Article em En | LILACS-Express | LILACS, VETINDEX | ID: biblio-1469558

Biblioteca responsável: BR68.1

ABSTRACT

ABSTRACT

A prospective analysis of active Human Cytomegalovirus infection (HCMV) was conducted on 33 pediatric renal or hematopoietic stem cell post-transplant patients. The HCMV-DNA positive samples were evaluated for the prevalence of different gB subtypes and their subsequent correlation with clinical signs. The surveillance of HCMV active infection was based on the monitoring of antigenemia (AGM) and on a nested polymerase chain reaction (N-PCR) for the detection of HCMV in the patients studied. Using restriction analysis of the gB gene sequence by PCR-RFLP (Restriction Fragment Length Polymorphism), different HCMV strains could be detected and classified in at least four HCMV genotypes. Thirty-three pediatric recipients of renal or bone marrow transplantation were monitored. Twenty out of thirty-three (60.6%) patients demonstrated active HCMV infection. gB1 and gB2 genotypes were more frequent in this population. In this study, we observed that gB2 had correlation with reactivation of HCMV infection and that patients with mixture of genotypes did not show any symptoms of HCMV disease. Future studies has been made to confirm this.

Palavras-chave

Antigenemia; Cytomegalovirus; GB genotypes; PCR; Pediatric transplantation

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: VETINDEX / LILACS Tipo de estudo: Risk_factors_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz. J. Microbiol. / Braz. j. microbiol Assunto da revista: MICROBIOLOGIA Ano de publicação: 2010 Tipo de documento: Article País de publicação: Brasil

Texto completo

Adicionar na Minha BVS

Imprimir

XML

Buscar no Google