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IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: cross-reaction with venoms from Bothrops species from Argentina
Rodríguez, JP; De Marzi, MC; Maruñak, S; Teibler, P; Acosta, O; Malchiodi, EL; Leiva, LC.
Afiliação
  • Rodríguez, JP; National University of the Northeast School of Natural and Exact Sciences and Land Surveying Department of Chemical Biology.
  • De Marzi, MC; University of Buenos Aires School of Pharmacy and Biochemistry Center for the Study of Humoral Immunity.
  • Maruñak, S; National University of the Northeast School of Veterinary Science Department of Pharmacology.
  • Teibler, P; National University of the Northeast School of Veterinary Science Department of Pharmacology.
  • Acosta, O; National University of the Northeast School of Veterinary Science Department of Pharmacology.
  • Malchiodi, EL; University of Buenos Aires School of Pharmacy and Biochemistry Center for the Study of Humoral Immunity.
  • Leiva, LC; National University of the Northeast School of Natural and Exact Sciences and Land Surveying Department of Chemical Biology.
J. Venom. Anim. Toxins incl. Trop. Dis. ; 15(3)2009.
Article em En | VETINDEX | ID: vti-443323
Biblioteca responsável: BR68.1
ABSTRACT
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
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Texto completo: 1 Base de dados: VETINDEX País/Região como assunto: America do sul / Argentina Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2009 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Base de dados: VETINDEX País/Região como assunto: America do sul / Argentina Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2009 Tipo de documento: Article