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A novel Kv1.1 potassium channel blocking toxin from the venom of Palamneus gravimanus (Indian black scorpion)
S. More, S.; K. Mirajkar, K.; R. Gadag, J.; S. Menon, K.; K. Mathew, M..
Afiliação
  • S. More, S.; Karnataka University Toxinology Division Department of Biochemistry.
  • K. Mirajkar, K.; Karnataka University Toxinology Division Department of Biochemistry.
  • R. Gadag, J.; Karnataka University Toxinology Division Department of Biochemistry.
  • S. Menon, K.; UAS-GKVK Tata Institute of Fundamental Research National Centre for Biological Sciences.
  • K. Mathew, M.; UAS-GKVK Tata Institute of Fundamental Research National Centre for Biological Sciences.
J. Venom. Anim. Toxins incl. Trop. Dis. ; 11(3)2005.
Article em En | VETINDEX | ID: vti-443034
Biblioteca responsável: BR68.1
ABSTRACT
A peptide toxin was isolated from the venom of Palamneus gravimanus, the Indian black scorpion, to block human Kv1.1 channels expressed in Xenopus laevis oocytes. A 4.5 kD peptide (toxin), as confirmed by SDS-PAGE, was purified to homogeneity by ion exchange chromatography using CM-Sephadex C-25 followed by Sephadex G-50 gel filtration. Palamneus gravimanus toxin (PGT) selectively blocks the human cloned voltage-gated potassium channel hKv1.1 in a two-electrode voltage-clamp (TEVC) technique. The results obtained indicate that the toxin blocks the hKv1.1 channel at a nanomolar concentration range (Ki value of 10 nM) of the peptide to the external side of the cell. The blockage seems to be voltage-dependent. Comparative structure of PGT (a 4.5 kD peptide) with BTK-2 suggests a close relationship; therefore this toxin can be employed to investigate the hKv1.1 channel structure.
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Texto completo: 1 Coleções: 01-internacional Base de dados: VETINDEX / LILACS Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. / J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2005 Tipo de documento: Article País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: VETINDEX / LILACS Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. / J. venom. anim. toxins incl. trop. dis Assunto da revista: TOXICOLOGIA Ano de publicação: 2005 Tipo de documento: Article País de publicação: Brasil