Toxicity of puffer fish: two species (Lagocephalus laevigatus, linaeus 1766 and Sphoeroides spengleri, Bloch 1785) from the Southeastern Brazilian coast

S. Oliveira, J.; R. Pires Junior, O.; A. V. Morales, R.; Bloch Junior, C.; A. Schwartz, C.; C. Freitas, J.

S. Oliveira, J.; R. Pires Junior, O.; A. V. Morales, R.; Bloch Junior, C.; A. Schwartz, C.; C. Freitas, J..

Afiliação

S. Oliveira, J.; University of São Paulo Institute of Biosciences and Marine Biology Center Department of Physiology.
R. Pires Junior, O.; University of Brasilia Department of Physiological Sciences Laboratory of Toxinology.
A. V. Morales, R.; University of Brasilia Department of Physiological Sciences Laboratory of Toxinology,Embrapa National Center of Genetic Resources and Biotechnology.
Bloch Junior, C.; Embrapa National Center of Genetic Resources and Biotechnology.
A. Schwartz, C.; University of Brasilia Department of Physiological Sciences Laboratory of Toxinology.
C. Freitas, J.; University of São Paulo Institute of Biosciences and Marine Biology Center Department of Physiology.

J. Venom. Anim. Toxins incl. Trop. Dis. ; 9(1)2003.

Article em En | VETINDEX | ID: vti-442927

Biblioteca responsável: BR68.1

ABSTRACT

ABSTRACT

In Brazil, where puffer fish are considered poisonous, there are few documented cases on human consumption and consequent poisoning. In this study, toxicity of two puffer fish species from the Brazilian coast was examined. Specimens of Sphoeroides spengleri and Lagocephalus laevigatus were caught in São Sebastião Channel (North coast of São Paulo State, Brazil) between January 1996 and May 1997. Acidic ethanol extracts from muscle and skin plus viscera were tested for mice acute toxicity using the standard method of Kawabata. Polar extracts of S. spengleri showed high toxicity up to 946 MU/g. Extracts from L. laevigatus showed very low levels of toxicity, never exceeding 1.7 MU/g. All extracts from both species blocked amielinic nerve fiber evoked impulses of crustacean legs; this effect reverted on washing similar to the standard tetrodotoxin TTX. The aqueous extract solutions were partially purified using an ionic exchange column (Amberlit GC-50) followed by treatment with activated charcoal (Norit-A). The presence of TTX and their analogs in the semi-purified extracts were confirmed by HPLC and mass spectrometry (MALDI-TOF).

Palavras-chave

puffer fish; Brazilian puffer; tetrodotoxin (TTX); envenoming; HPLC and mass spectrometry

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Texto completo: 1 Base de dados: VETINDEX País/Região como assunto: America do sul / Brasil Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2003 Tipo de documento: Article

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