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Rapid PCR-based assay for Sclerotinia sclerotiorum detection on soybean seeds
Grabicoski, Edilaine Mauricia Gelinski; Jaccoud Filho, David de Souza; Pileggi, Marcos; Henneberg, Luciane; Pierre, Marcelo Luiz Cunha; Vrisman, Cláudio Mauricio; Dabul, Audrei Nisio Gebieluca.
Afiliação
  • Grabicoski, Edilaine Mauricia Gelinski; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Jaccoud Filho, David de Souza; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Pileggi, Marcos; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Henneberg, Luciane; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Pierre, Marcelo Luiz Cunha; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Vrisman, Cláudio Mauricio; State University of Ponta Grossa. Ponta Grossa. Brazil
  • Dabul, Audrei Nisio Gebieluca; State University of Ponta Grossa. Ponta Grossa. Brazil
Sci. agric. ; 72(1): 69-74, Jan.-Feb. 2015. ilus, tab
Article em En | VETINDEX | ID: vti-30059
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Caused by Sclerotinia sclerotiorum, white mold is an important seed-transmitted disease of soybean (Glycine max). Incubation-based methods available for the detection and quantification of seed-borne inoculum such as the blotter test, paper roll and Neon-S assay are time-consuming, laborious, and not always sensitive. In this study, we developed and evaluated a molecular assay for the detection of S. sclerotiorum in soybean seeds using a species-specific PCR (polymerase chain reaction) primer set and seed soaking (without DNA extraction) for up to 72 h. The PCR products were amplified in all the samples infected with the pathogen, but not in the other samples of plant material or the other seed-borne fungi DNA. The minimum amount of DNA detected was 10 pg, or one artificially infested seed in a 400-seed sample (0.25 % fungal incidence) and one naturally infected seed in a 300-seed sample (0.33 % incidence). The PCR-based assay was rapid ( 9 h), did not require DNA extraction and was very sensitive.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Doenças das Plantas / Ascomicetos / Glycine max / Reação em Cadeia da Polimerase Idioma: En Revista: Sci. agric / Sci. agric. Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Doenças das Plantas / Ascomicetos / Glycine max / Reação em Cadeia da Polimerase Idioma: En Revista: Sci. agric / Sci. agric. Ano de publicação: 2015 Tipo de documento: Article