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Detection of Ehrlichia canis in domestic cats in the central-western region of Brazil
Braga, Ísis Assis; Santos, Luana Gabriela Ferreira dos; Ramos, Dirceu Guilherme de Souza; Melo, Andréia Lima Tomé; Mestre, Gustavo Leandro da Cruz; Aguiar, Daniel Moura de.
Afiliação
  • Braga, Ísis Assis; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
  • Santos, Luana Gabriela Ferreira dos; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
  • Ramos, Dirceu Guilherme de Souza; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
  • Melo, Andréia Lima Tomé; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
  • Mestre, Gustavo Leandro da Cruz; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
  • Aguiar, Daniel Moura de; Universidade Federal de Mato Grosso. Faculdade de Agronomia, Medicina Veterinária e Zootecnia. Cuiabá. BR
Braz. J. Microbiol. ; 45(2): 641-645, Apr.-June 2014. mapas
Article em En | VETINDEX | ID: vti-27322
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Ehrlichiosis is a worldwide distributed disease caused by different bacteria of the Ehrlichia genus that are transmitted by arthropod vectors. Its occurrence in dogs is considered endemic in several regions of Brazil. Regarding cats, however, few studies have been done and, consequently, there is not enough data available. In order to detect Ehrlichia spp. in cats from the central-western region of Brazil, blood and serum samples were collected from a regional population of 212 individuals originated from the cities of Cuiabá and Várzea Grande. These animals were tested by the Immunofluorescence Assay (IFA) and the Polymerase Chain Reaction (PCR) designed to amplify a 409 bp fragment of the dsb gene. The results obtained show that 88 (41.5%) cats were seropositive by IFA and 20 (9.4%) cats were positive by PCR. The partial DNA sequence obtained from PCR products yielded twenty samples that were found to match perfectly the Ehrlichia canis sequences deposited on GenBank. The natural transmission of Ehrlichia in cats has not been fully established. Furthermore, tick infestation was not observed in the evaluated cats and was not observed any association between age, gender and positivity of cats in both tests. The present study reports the first serological and molecular detection of E. canis in domestic cats located in the endemic area previously mentioned.
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Doenças do Gato / Ehrlichiose / Ehrlichia canis Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2014 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Doenças do Gato / Ehrlichiose / Ehrlichia canis Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2014 Tipo de documento: Article