Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model
Acta cir. bras.
; 34(4): e201900403, May 2019. tab, graf, ilus
Article
em En
| VETINDEX
| ID: vti-23229
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Purpose:
To investigate the long non-coding RNAs (lncRNAs) profile on renal ischemia reperfusion in a mouse model.Methods:
Microarray analysis was used to study the expression of misregulated lncRNA in a mouse model of renal ischemia reperfusion (I/R) with long ischemia time. Quantitative real-time PCR (qPCR) was used to verify the expression of selected lncRNAs and mRNAs. The potential functions of the lncRNA was analyzed by bioinformatics tools and databases.Results:
Kidney function was impaired in I/R group compared to the normal group. Analysis showed that a total of 2267 lncRNAs and 2341 messenger RNAs (mRNAs) were significantly expressed in I/R group (≥2.0-fold, p < 0.05). The qPCR result showed that lncRNAs and mRNAs expression were consistent with the microarray analysis. The co-expression network profile analysis based on five validated lncRNAs and 203 interacted mRNAs showed it existed a total of 208 nodes and 333 connections. The GO and KEEG pathway analysis results showed that multiple lncRNAs are involved the mechanism of I/R.Conclusion:
Multiple lncRNAs are involved in the mechanism of I/R. These analysis results will help us to further understand the mechanism of I/R and promote the new methods targeted at lncRNA to improve I/R injury.(AU)Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
RNA Mensageiro
/
Reperfusão
/
RNA Longo não Codificante
/
Isquemia
/
Rim
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras.
Ano de publicação:
2019
Tipo de documento:
Article