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Expression analysis of long non-coding RNAs in a renal ischemia-reperfusion injury model
Tao, Qiu; Tianyu, Wang; Jiangqiao, Zhou; Zhongbao, Chen; Xiaoxiong, Ma; Long, Zhang; Jilin, Zou.
Afiliação
  • Tao, Qiu; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Tianyu, Wang; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Jiangqiao, Zhou; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Zhongbao, Chen; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Xiaoxiong, Ma; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Long, Zhang; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
  • Jilin, Zou; Wuhan University. Renmin Hospital. Department of Organ Transplantation. Hubei. China
Acta cir. bras. ; 34(4): e201900403, May 2019. tab, graf, ilus
Article em En | VETINDEX | ID: vti-23229
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Purpose:

To investigate the long non-coding RNAs (lncRNAs) profile on renal ischemia reperfusion in a mouse model.

Methods:

Microarray analysis was used to study the expression of misregulated lncRNA in a mouse model of renal ischemia reperfusion (I/R) with long ischemia time. Quantitative real-time PCR (qPCR) was used to verify the expression of selected lncRNAs and mRNAs. The potential functions of the lncRNA was analyzed by bioinformatics tools and databases.

Results:

Kidney function was impaired in I/R group compared to the normal group. Analysis showed that a total of 2267 lncRNAs and 2341 messenger RNAs (mRNAs) were significantly expressed in I/R group (≥2.0-fold, p < 0.05). The qPCR result showed that lncRNAs and mRNAs expression were consistent with the microarray analysis. The co-expression network profile analysis based on five validated lncRNAs and 203 interacted mRNAs showed it existed a total of 208 nodes and 333 connections. The GO and KEEG pathway analysis results showed that multiple lncRNAs are involved the mechanism of I/R.

Conclusion:

Multiple lncRNAs are involved in the mechanism of I/R. These analysis results will help us to further understand the mechanism of I/R and promote the new methods targeted at lncRNA to improve I/R injury.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: RNA Mensageiro / Reperfusão / RNA Longo não Codificante / Isquemia / Rim Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2019 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: RNA Mensageiro / Reperfusão / RNA Longo não Codificante / Isquemia / Rim Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2019 Tipo de documento: Article