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Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion
Bazzano, Telma; Restel, Tamy Ingrid; Porfirio, Lenir Cardoso; Souza, Albert Schiaveto de; Silva, Iandara Schettert.
Afiliação
  • Bazzano, Telma; Federal University of Mato Grosso do Sul. Postgraduate Program in Health and Development of the Midwest. Campo Grande. Brazil
  • Restel, Tamy Ingrid; Federal University of Mato Grosso do Sul. Central Animal Facility. Campo Grande. Brazil
  • Porfirio, Lenir Cardoso; Federal University of Espirito Santo. Department of Veterinary Medicine. Vitória. Brazil
  • Souza, Albert Schiaveto de; Federal University of Mato Grosso do Sul. Center for Biological and Health Sciences. Campo Grande. Brazil
  • Silva, Iandara Schettert; Federal University of Mato Grosso do Sul. Postgraduate Program in Health and Development of the Midwest. Campo Grande. Brazil
Acta cir. bras. ; 30(4): 277-288, Apr. 2015. ilus, tab
Article em En | VETINDEX | ID: vti-21996
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

PURPOSE:

To investigate biomarkers of acute renal injury in Wistar rats, subjected to left renal ischemia for 10 minutes, and then compare reperfusion at 24 hours, and at 5, 7, 14 and 21 days after the procedure.

METHODS:

Eight female and male rats between 60 and 81 days old were used in the Central Animal Facility of the UFMS. Assessed biomarkers included urine protein, urea, creatinine, glucose, sodium, potassium, urine alkaline phosphatase and gamma-glutamyl transferase activities, and protein-to-creatinine ratio; and in serum urea, creatinine, sodium and potassium, fractional excretion of sodium, potassium, urine flow and creatinine clearance.

RESULTS:

Greater variance was observed in the parameters at 24 hours and at five days (p 0.05) after reperfusion. On the 21st day, these parameters approximated those obtained for the control group.

CONCLUSIONS:

Renal ischemia for 10 minutes was sufficient to raise urine levels of protein, glucose, fractional excretion of potassium, urea, creatinine clearance, urine activity of gamma-glutamyltransferase and alkaline phosphatase enzymes in the first 24 hours, up to five days after reperfusion, which may indicate risk of acute kidney injury, according to the RIFLE classification.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Biomarcadores / Injúria Renal Aguda / Isquemia Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2015 Tipo de documento: Article / Project document

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Biomarcadores / Injúria Renal Aguda / Isquemia Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2015 Tipo de documento: Article / Project document