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Antileishmanial activity and immunomodulatory effect of secosubamolide, a butanolide isolated from Nectandra oppositifolia (Lauraceae)
Costa-Silva, Thais A. da; Conserva, Geanne A. Alves; Galisteo Junior, Andrés J; Tempone, Andre G; Lago, João Henrique G.
Afiliação
  • Costa-Silva, Thais A. da; Federal University of ABC. Center of Natural Sciences and Humanities. São Paulo. Brasil
  • Conserva, Geanne A. Alves; Federal University of ABC. Center of Natural Sciences and Humanities. São Paulo. Brasil
  • Galisteo Junior, Andrés J; University of São Paulo. Institute of Tropical Medicine. São Paulo. Brasil
  • Tempone, Andre G; Adolfo Lutz Institute. Centre for Parasitology and Mycology. São Paulo. Brasil
  • Lago, João Henrique G; Federal University of ABC. Center of Natural Sciences and Humanities. São Paulo. Brasil
J. Venom. Anim. Toxins incl. Trop. Dis. ; 25: e20190008, Aug. 19, 2019. ilus, tab, graf
Article em En | VETINDEX | ID: vti-21886
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

Background:

Visceral leishmaniasis is a complex neglected tropical disease caused by Leishmania donovani complex. Its current treatment reveals strong limitations, especially high toxicity. In this context, natural products are important sources of new drug alternatives for VL therapy. Therefore, the antileishmanial and immunomodulatory activity of compounds isolated from Nectandra oppositifolia (Lauraceae) was investigated herein.

Methods:

The n-hexane extract from twigs of N. oppositifolia were subjected to HPLC/HRESIMS and bioactivity-guided fractionation to afford compounds 1 and 2 which were evaluated in vitro against Leishmania (L.) infantum chagasi and NCTC cells.

Results:

The n-hexane extract displayed activity against L. (L.) infantum chagasi and afforded isolinderanolide E (1) and secosubamolide A (2), which were effective against L. (L.) infantum chagasi promastigotes, with IC50 values of 57.9 and 24.9 µM, respectively. Compound 2 was effective against amastigotes (IC50 = 10.5 µM) and displayed moderate mammalian cytotoxicity (CC50 = 42 µM). The immunomodulatory studies of compound 2 suggested an anti-inflammatory activity, with suppression of IL-6, IL-10, TNF with lack of nitric oxide.

Conclusion:

This study showed the antileishmanial activity of compounds 1 and 2 isolated from N. oppositifolia. Furthermore, compound 2 demonstrated an antileishmanial activity towards amastigotes associated to an immunomodulatory effect.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: 4-Butirolactona / Leishmaniose / Leishmania infantum / Lauraceae Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2019 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: 4-Butirolactona / Leishmaniose / Leishmania infantum / Lauraceae Idioma: En Revista: J. Venom. Anim. Toxins incl. Trop. Dis. Ano de publicação: 2019 Tipo de documento: Article