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Effects of perioperative hypothermia and reactive oxygen species in the healing of colonic anastomosis in rats
Oliveira, João Carlos Costa de; Oliveira, Camila Helena de; Oliveira, Henrique Eduardo de; Pereira, Aline; Maraschin, Marcelo; d'Acâmpora, Armando José.
Afiliação
  • Oliveira, João Carlos Costa de; Federal University of Santa Catarina (UFSC). Department of Surgery. Florianópolis. Brazil
  • Oliveira, Camila Helena de; University of Southern Santa Catarina (UNISUL). Palhoça. Brazil
  • Oliveira, Henrique Eduardo de; University of Southern Santa Catarina (UNISUL). Palhoça. Brazil
  • Pereira, Aline; Federal University of Santa Catarina (UFSC). Center for Agricultural Sciences. Brazil Plant Morphogenesis and Biochemistry Laboratory. Florianópolis. Brazil
  • Maraschin, Marcelo; Federal University of Santa Catarina (UFSC). Center for Agricultural Sciences. Brazil Plant Morphogenesis and Biochemistry Laboratory. Florianópolis. Brazil
  • d'Acâmpora, Armando José; Federal University of Santa Catarina (UFSC). Department of Surgery. Florianópolis. Brazil
Acta cir. bras. ; 29(11): 742-747, Nov. 2014. ilus, graf
Article em En | VETINDEX | ID: vti-21364
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

PURPOSE:

We evaluated the hypothesis that induced perioperative hypothermia (32 ± 1ºC) affects the redox balance in the tissue of colonic anastomosis in rats by modifying biochemical enzymatic and non-enzymatic markers related to oxidative stress.

METHODS:

Forty-eight male Wistar rats were randomly divided into eight experimental groups of six animals each and underwent laparotomy, sigmoid section and immediate anastomosis. Four groups were operated under normothermia (36 ± 1ºC), and the other four under hypothermia (32 ± 1ºC). The animals were reoperated on days 3, 7 and 14 postoperatively, and two groups underwent SHAM at 3 days. From the scar tissue samples, the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was evaluated, and the levels of non-enzymatic markers of oxidative stress, such as reduced glutathione (GSH) and lipid peroxidation, were measured by the thiobarbituric acid reactive substances (TBARS) assay. The means were compared between groups corresponding to each day of sampling and euthanasia.

RESULTS:

The hypothermic groups showed a significant reduction on the activity of SOD on day 7 postoperatively, on the activity of CAT on days 7 and 14 postoperatively and on the levels of GSH on day 7 postoperatively. The level of lipid peroxidation was increased in the hypothermia group on day 7 postoperatively and decreased on day 14 compared with the normothermic groups.

CONCLUSION:

Perioperative hypothermia reduced the activity of the antioxidant enzymes catalase and superoxide dismutase, glutathione levels and increased lipid peroxidation in the scar tissue of colonic anastomoses in rats.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Cicatrização / Anastomose Cirúrgica / Espécies Reativas de Oxigênio / Período Perioperatório / Hipotermia Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Cicatrização / Anastomose Cirúrgica / Espécies Reativas de Oxigênio / Período Perioperatório / Hipotermia Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2014 Tipo de documento: Article