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Pentoxifylline associated to hypertonic saline solution attenuates inflammatory process and apoptosis after intestinal ischemia/reperfusion in rats
Marques, Geraldo Magela Nogueira; Rasslan, Roberto; Belon, Alessandro Rodrigo; Carvalho, Juliana Gonçalves; Felice Neto, Raphael; Rasslan, Samir; Utiyama, Edivaldo Massazo; Montero, Edna Frasson de Souza.
Afiliação
  • Marques, Geraldo Magela Nogueira; São Paulo University (USP). Medical School. Laboratory of Surgical Physiopathology (LIM-62); Postgraduate Program in Surgical Clinics. São Paulo. Brazil
  • Rasslan, Roberto; São Paulo University (USP). Medical School. Laboratory of Surgical Physiopathology; Program in Surgical Clinics. São Paulo. Brazil
  • Belon, Alessandro Rodrigo; São Paulo University (USP). Medical School. Laboratory of Experimental Surgery (LIM-26). São Paulo. Brazil
  • Carvalho, Juliana Gonçalves; Federal University of Sao Paulo (UNIFESP). Medical School. Postgraduate Program in Pathology. São Paulo. Brazil
  • Felice Neto, Raphael; São Paulo University (USP). School of Medicine. São Paulo. Brazil
  • Rasslan, Samir; São Paulo University (USP). School of Medicine. Department of Surgery. São Paulo. Brazil
  • Utiyama, Edivaldo Massazo; São Paulo University (USP). Medical School. Department of Surgery. São Paulo. Brazil
  • Montero, Edna Frasson de Souza; São Paulo University (USP). Medical School. Department of Surgery; Laboratory of Surgical Research (LIM-62). São Paulo. Brazil
Acta cir. bras. ; 29(11): 735-741, Nov. 2014. tab, ilus
Article em En | VETINDEX | ID: vti-21342
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT

PURPOSE:

To evaluate intestinal inflammatory and apoptotic processes after intestinal ischemia/reperfusion injury, modulated by pentoxifylline and hypertonic saline.

METHODS:

It was allocated into four groups (n=6), 24 male Wistar rats (200 to 250g) and submitted to intestinal ischemia for 40 min and reperfusion for 80 min IR (did not receive any treatment); HS group (Hypertonic Saline, 4ml/kg-IV); PTX group (Pentoxifylline, 30mg/kg-IV); HS+PTX group (Hypertonic Saline and Pentoxifylline). All animals were heparinized (100U/kg). At the end of reperfusion, ileal fragments were removed and stained on hematoxylin-eosin and histochemical studies for COX-2, Bcl-2 and cleaved caspase-3.

RESULTS:

The values of sO2 were higher on treated groups at 40 minutes of reperfusion (p=0.0081) and 80 minutes of reperfusion (p=0.0072). Serum lactate values were lower on treated groups after 40 minutes of reperfusion (p=0.0003) and 80 minutes of reperfusion (p=0.0098). Morphologic tissue injuries showed higher grades on IR group versus other groups HS (p=0.0006), PTX (p=0.0433) and HS+PTX (p=0.0040). The histochemical study showed lesser expression of COX-2 (p=0.0015) and Bcl-2 (p=0.0012) on HS+PTX group. A lower expression of cleaved caspase-3 was demonstrated in PTX (p=0.0090; PTXvsIR).

CONCLUSION:

The combined use of pentoxifylline and hypertonic saline offers best results on inflammatory and apoptotic inhibitory aspects after intestinal ischemia/reperfusion.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Pentoxifilina / Solução Salina Hipertônica / Ratos Wistar / Apoptose / Inflamação Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2014 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Pentoxifilina / Solução Salina Hipertônica / Ratos Wistar / Apoptose / Inflamação Limite: Animals Idioma: En Revista: Acta cir. bras. Ano de publicação: 2014 Tipo de documento: Article