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Oocyte maturation with royal jelly increases embryo development and reduces apoptosis in goats
Veshkini, Arash; Mohammadi-Sangcheshmeh, Abdollah; Ghanem, Nasser; Abazari-kia, Amir Hossein; Mottaghi, Elmira; Kamaledini, Reza; Deldar, Hamid; Ozturk, Irfan; Gastal, Eduardo Leite.
Afiliação
  • Veshkini, Arash; Stem Cell Technology Research Center. Department of Transgenic Animal Science. Tehran. IR
  • Mohammadi-Sangcheshmeh, Abdollah; University of Tehran. College of Aburaihan. Department of Animal and Poultry Science. Tehran. IR
  • Ghanem, Nasser; Cairo University. Faculty of Agriculture. Department of Animal Production. Giza. EG
  • Abazari-kia, Amir Hossein; Stem Cell Technology Research Center. Department of Transgenic Animal Science. Tehran. IR
  • Mottaghi, Elmira; Islamic Azad University. Tehran Science and Research Branch. Faculty of Agriculture and Natural Resources; Department of Animal Science and Technology. Tehran. IR
  • Kamaledini, Reza; Islamic Azad University. Tehran Science and Research Branch. Department of Animal Science and Technology; Faculty of Agriculture and Natural Resources. Tehran. IR
  • Deldar, Hamid; Sari Agricultural Sciences and Natural Resources University. College of Animal Science and Fisheries. Department of Animal Science. Sari. IR
  • Ozturk, Irfan; Harran University. Faculty of Agriculture. Department of Biometry Science. Sanliurfa. TR
  • Gastal, Eduardo Leite; Southern Illinois University. Food and Nutrition. Department of Animal Science. Carbondale. US
Anim. Reprod. (Online) ; 15(2): 124-134, Apr.-June.2018. ilus, graf, tab
Article em En | VETINDEX | ID: biblio-1461349
Biblioteca responsável: BR68.1
Localização: BR68.1
ABSTRACT
Royal jelly (RJ) was supplemented to goat oocytein vitro maturation (IVM) medium at three different concentrations (2.5, 5, and 10 mg/ml). Maturation rate, embryo cleavage, and blastocyst rate were recorded. Gene expression of apoptosis-related transcripts was investigated in matured oocytes. Percentage of oocytes that reached MII-stage was increased in RJ-treated groups compared to the control group. Glutathione (GSH) content of mature oocytes was enhanced when RJ was added to IVM medium at any supplementation compared with control. Percentage of cleaved embryos and blastocysts was higher in the RJ-treated groups at a concentration of 5 mg/ml than in the 2.5 mg/ml and control group. Total number of cells per blastocyst was not different in the control and RJ-treated group at 5 mg/ml. However, number of apoptotic cells per blastocyst was higher in the control group than in the RJ-treated group at 5 mg/ml. Expression profile of Bax, and p53was down-regulated while Bcl-2 was up-regulated in oocytes treated with RJ at 5 and 10 mg/ml compared with the control group. Addition of RJ at concentrations of 5 mg/ml improved embryo production through increasing maturation rate. RJ seems to improvethe IVM microenvironment by reducing expression of genes inducing apoptosis, enhancing GSH content, and reducing incidence of apoptosis in blastocysts.
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Ruminantes / Desenvolvimento Embrionário Limite: Animals Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2018 Tipo de documento: Article / Project document
Texto completo
Adicionar na Minha BVS
Imprimir
XML
Buscar no Google

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Ruminantes / Desenvolvimento Embrionário Limite: Animals Idioma: En Revista: Anim. Reprod. / Anim. Reprod. (Online) Ano de publicação: 2018 Tipo de documento: Article / Project document