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Simultaneous biodegradation of phenol and cyanide present in coke-oven effluent using immobilized Pseudomonas putida and Pseudomonas stutzeri
Singh, Utkarsh; Arora, Naveen Kumar; Sachan, Preeti.
Afiliação
  • Singh, Utkarsh; Chhatrapati Shahu Ji Maharaj University. Department of Microbiology. Institute of Biosciences and Biotechnology. Uttar Pradesh. Índia
  • Arora, Naveen Kumar; Babasaheb Bhimrao Ambedkar University. Department of Environmental Microbiology. Uttar Pradesh. Índia
  • Sachan, Preeti; Gurukula Kangri University. Kanya Gurukul Campus. Department of Environmental Sciences. Uttarakhand. Índia
Braz. J. Microbiol. ; 49(1): 38-44, jan.-mar. 2018. tab, graf
Article em En | VETINDEX | ID: vti-18754
Biblioteca responsável: BR68.1
ABSTRACT
Discharge of coke-oven wastewater to the environment may cause severe contamination to it and also threaten the flora and fauna, including human beings. Hence before dumping it is necessary to treat this dangerous effluent in order to minimize the damage to the environment. Conventional technologies have inherent drawbacks however, biological treatment is an advantageous alternative method. In the present study, bacteria were isolated from the soil collected from the sites contaminated by coke-oven effluent rich in phenol and cyanide. Nucleotides sequence alignment and phylogenetic analysis showed the identity of the selected phenol and cyanide degrading isolates NAUN-16 and NAUN-1B as Pseudomonas putida and Pseudomonas stutzeri, respectively. These two isolates tolerated phenol up to 1800 mg L-1 and cyanide up to 340 mg L-1 concentrations. The isolates were immobilized on activated charcoal, saw dust and fly ash. The effluent was passed through the column packed with immobilized cells with a flow rate of 5 mL min-1. The isolates showed degradation of phenol up to 80.5% and cyanide up to 80.6% and also had the ability to reduce biological oxygen demand, chemical oxygen demand and lower the pH of effluent from alkaline to near neutral. The study suggests the utilization of such potential bacterial strains in treating industrial effluent containing phenol and cyanide, before being thrown in any ecosystem.(AU)
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Texto completo: 1 Base de dados: VETINDEX Assunto principal: Biodegradação Ambiental / Pseudomonas putida / Coque / Fenol / Cianetos / Pseudomonas stutzeri País/Região como assunto: Asia Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2018 Tipo de documento: Article

Texto completo: 1 Base de dados: VETINDEX Assunto principal: Biodegradação Ambiental / Pseudomonas putida / Coque / Fenol / Cianetos / Pseudomonas stutzeri País/Região como assunto: Asia Idioma: En Revista: Braz. J. Microbiol. Ano de publicação: 2018 Tipo de documento: Article