Inside-out and standard vein grafts associated with platelet-rich plasma (PRP) in sciatic nerve repair. A histomorphometric study
Acta cir. bras.
; 32(8): 617-625, Aug. 2017. ilus, tab
Article
em En
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| ID: vti-17720
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Purpose:
To evaluated the tubulization technique with standard and inside-out vein, filled or not with platelet-rich plasma (PRP), in sciatic nerve repair.Methods:
Seventy male Wistar rats were randomly divided into five groups IOVNF (Inside-Out Vein with No Filling); IOVPRP (Inside-Out Vein filled with PRP); SVNF (Standard Vein with No Filling); SVPRP (Standard Vein filled with PRP); Sham (Control). The left external jugular vein was used as graft in a 10 mm nervous gap.Results:
In the morphological analysis of all groups, myelinated nerve fibers with evident myelin sheath, neoformation of the epineurium and perineurium, organization of intraneural fascicles and blood vessels were observed. In the morphometry of the distal stump fibers, SVPRP group had the highest means regarding fiber diameter (3.63±0.42 m), axon diameter (2.37±0.31 m) and myelin sheath area (11.70±0.84 m2). IOVPRP group had the highest means regarding axon area (4.39±1.16 m2) and myelin sheath thickness (0.80±0.19 m). As for values of the fiber area, IOVNF group shows highest means (15.54±0.67 m2), but are still lower than the values of the Sham group.Conclusion:
The graft filled with platelet-rich plasma, with use standard (SVPRP) or inside-out vein (IOVPRP), promoted the improvement in axonal regeneration on sciatic nerve injury.(AU)Palavras-chave
Texto completo:
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Base de dados:
VETINDEX
Assunto principal:
Ratos
/
Plasma Rico em Plaquetas
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras.
Ano de publicação:
2017
Tipo de documento:
Article