UltramicroELISA for measuring tetanus antitoxin in human sera
Bulletin of the Pan American Health Organization (PAHO)
; 30(1),mar. 1996 ilus
Article
| PAHOIRIS
| ID: phr-27782
Biblioteca responsável:
US1.1
ABSTRACT
This article describes a combination of methods- a solid-phase enzyme-linked immunosorbent assay (ELISA) combined with an ultramicroanalytical system (UMAS)- that can be used to measure tetanus antitoxin activity in human sera or plasma. The test, which is rapid and permits analysis of 78 samples of serum per reaction plate with a volume of 10 uL of diluted serum per sample, is proposed as an alternative to the traditional biologic assay in mice based on seroneutralization of a known dose of tetanus toxin. The study reported here compared these two procedures, using them both to evaluate 100 sera from de Clinical Laboratory of the General Calixto García Hospital in Havana, Cuba. The two sets of results showed a high degree of correlation (r = 0.99) when subjected to linear regression analysis (95 percent CI = 0.985-0.993). These and other findings indicate that the cheap and rapid ultramicroELISA method can perform certain tasks for which the slower and costlier traditional assay is not well suited, such as field evaluation of tetanus toxoid vaccines and identification of hyperimmune plasmas appropriate for use in producing specific antitetanus immunoglobulin (AU)
Texto completo:
1
Coleções:
04-international_org
Base de dados:
PAHOIRIS
Assunto principal:
Bioensaio
/
Ensaio de Imunoadsorção Enzimática
/
Antitoxina Tetânica
/
Técnicas Imunoenzimáticas
/
Cuba
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Caribe
/
Cuba
Ano de publicação:
1996
Tipo de documento:
Article