Evaluation of a membrane (Nuclepore) filtration method using capillary blood for the detection of microfilariae
Trop Med Parasitol
; 76(3): 339-45, 1982.
Article
em En
| MedCarib
| ID: med-9413
Biblioteca responsável:
JM3.1
Localização: JM3.1; Reprint Collection
ABSTRACT
The efficacy of a membrane (Nuclepore) filtration technique using capillary blood (100 mm 3) for the detection of blood-circulating microfilariae was investigated during a prevalence survey for Wuchereria bancrofti and Mansonella ozzardi in north Trinidad by comparing results thus obtained with results from conventional thick smear preparations (25mm3) and from membrane (Nuclepore) filtration of venous blood (1 ml). Prevalence rates of W. bancrofti were 14 percent by thick smear, 17 percent by filtration of capillary blood and 6 percent by filtratioon of venous blood. For M. ozzardi the corresponding rates were 27 percent, 29 percent and 31 percent. A total of 389 persons was examined by all three methods. Microfilaria detection rates for both of the filtration techniques were significantly higher than by thick smear preparation, with the exception of results for M. ozzardi obtained by filtration of capillary blood. There was no significant difference between the number of positive persons detected by filtration of capillary blood and filtration of venous blood. Significantly higher microfilaria densities per unit volume of blood were recorded by thick smear preparation and filtration of capillary blood than by filtration of venous blood. Whereas results for the two capillary blood techniques were similar. In the detection of low density microfilaraemias, membrane filtration of capillary blood provides a practical and sensitive alternative to venous blood filtration and has the additional advantage of capilary blood collection rather than venepuncture.(AU)
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Coleções:
01-internacional
Base de dados:
MedCarib
Assunto principal:
Sangue
/
Filariose
Tipo de estudo:
Diagnostic_studies
/
Risk_factors_studies
Limite:
Child
/
Humans
País/Região como assunto:
Caribe ingles
/
Trinidad y tobago
Idioma:
En
Revista:
Trop Med Parasitol
Ano de publicação:
1982
Tipo de documento:
Article