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HIV-1 epidemic in the Caribbean is dominated by subtype B.
Nadai, Yuka ; Eyzaguirre, Lindsay M. ; Sill, Anne ; Cleghorn, Farley ; Nolte, Claudine ; Charurat, Manhattan ; Collado-Chastel, Santiago ; Jack, Noreen ; Bartholomew, Courtenay ; Pape, Jean W. ; Figueroa, Peter ; Blattner, William A. ; Carr, Jean K. .
Afiliação
  • Nadai, Yuka ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Eyzaguirre, Lindsay M. ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Sill, Anne ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Cleghorn, Farley ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Nolte, Claudine ; Cornell University. Weill Medical College. New York. United States of America
  • Charurat, Manhattan ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Collado-Chastel, Santiago ; Institute of Biomedical Studies. Santo Domingo. Dominican Republic
  • Jack, Noreen ; Medical Research Foundation. Port of Spain. Trinidad and Tobago
  • Bartholomew, Courtenay ; Medical Research Foundation. Port of Spain. Trinidad and Tobago
  • Pape, Jean W. ; Cornell University. Weill Medical College. New York. United States of America
  • Figueroa, Peter ; Ministry of Health. Kingston. Jamaica
  • Blattner, William A. ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
  • Carr, Jean K. ; University of Maryland. Institute of Human Virology. School of Medicine. Baltimore, Maryland. United States of America
PLos One ; 4(3): [1-5], mar. 2009. tabilus
Article em En | MedCarib | ID: med-17878
Biblioteca responsável: TT5
Localização: TT5
ABSTRACT

BACKGROUND:

The molecular epidemiology of HIV-1 in the Caribbean has been described using partial genome sequencing; subtype B is the most common subtype in multiple countries. To expand our knowledge of this, nearly full genome amplification, sequencing and analysis was conducted. METHODOLOGY/PRINCIPAL

FINDINGS:

Virion RNA from sera collected in Haiti, Dominican Republic, Jamaica and Trinidad and Tobago were reverse transcribed, PCR amplified, sequenced and phylogenetically analyzed. Nearly full genomes were completed for 15 strains; partial pol was done for 67 strains. All but one of the 67 strains analyzed in pol were subtype B; the exception was a unique recombinant of subtypes B and C collected in the Dominican Republic. Of the nearly full genomes of 14 strains that were subtype B in pol, all were subtype B from one end of the genome to the other and not inter-subtype recombinants. Surprisingly, the Caribbean subtype B strains clustered significantly with each other and separate from subtype B from other parts of the pandemic.

CONCLUSIONS:

The more complete analysis of HIV-1 from 4 Caribbean countries confirms previous research using partial genome analysis that the predominant subtype in circulation was subtype B. The Caribbean strains are phylogenetically distinct from other subtype B strains although the biological meaning of this finding is unclear.
Assuntos
Texto completo: 1 Coleções: 01-internacional Base de dados: MedCarib Assunto principal: Trinidad e Tobago / Genoma Humano / HIV-1 / Região do Caribe / República Dominicana / Haiti / Jamaica Limite: Humans País/Região como assunto: Caribe / Caribe ingles / Haiti / Jamaica / Republica dominicana / Trinidad y tobago Idioma: En Revista: PLos One Ano de publicação: 2009 Tipo de documento: Article
Texto completo: 1 Coleções: 01-internacional Base de dados: MedCarib Assunto principal: Trinidad e Tobago / Genoma Humano / HIV-1 / Região do Caribe / República Dominicana / Haiti / Jamaica Limite: Humans País/Região como assunto: Caribe / Caribe ingles / Haiti / Jamaica / Republica dominicana / Trinidad y tobago Idioma: En Revista: PLos One Ano de publicação: 2009 Tipo de documento: Article