Detection of Mycobacterium bovis in milk by polymerase chain reaction.
Zentralbl Veterinarmed B
; 45(8): 473-9, 1998 Oct.
Article
em En
| MEDLINE
| ID: mdl-9820115
A simple method was developed for DNA extraction of Mycobacterium bovis in milk and further detection of the bacterium by polymerase chain reaction (PCR). Milk previously seeded with M. bovis was used as the starting material. The procedure involved overnight digestion of a milk sample with proteinase K at 56 degrees C and phenol extraction, followed by ethanol precipitation and PCR. The amplification pattern obtained was analyzed with primers BW8-BW9 which amplify a 248 bp in strains of M. bovis. By using the BW8-BW9 primers, 10(3) CFU were detected on silver-stained PAGE gels. The procedure was validated by PCR analysis of milk in tuberculin-positive animals. It is anticipated that this method can be used for routine diagnosis of M. bovis in milk samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Leite
/
Mycobacterium bovis
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Zentralbl Veterinarmed B
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Alemanha