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[Antibody radiolabeling with technetium-99m]. / Radiomarcaje de anticuerpos con Tecnecio-99m.
Perera, A; Pérez, C.
Afiliação
  • Perera A; Centro de Investigaciones Clínicas, Kohly. Playa, Ciudad de La Habana, 11300, Cuba. cic@cic.sld.cu
Rev Esp Med Nucl ; 17(4): 302-9, 1998.
Article em Es | MEDLINE | ID: mdl-9721347
Nowadays, labelled polyclonal and monoclonal antibodies are widely used for immunoscintigraphic diagnosis of different diseases. Technetium-99m is often considered to be the label of choice for radioimmunodiagnosis for reasons of cost, availability and imaging properties, in spite of its relatively short physical half-life (6.01 h). The existing labelling methods may be classified into two types: direct approaches, in which disulphide bridges within are reduced to generate endogenous sulfhydryl groups able to efficiently bind technetium due to their strong chelating capacity and indirect methods, in which an exogenous chelator is covalently attached to the protein to serve as the binding site. All these procedures have their advantages and drawbacks. There is no consensus among the authors about which of the methods is the best. The employed approach depends on the particular situation. The aim of the present work is to show an update about the available procedures for 99mTc-labelling of antibodies and its fragments.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Imunoglobulinas / Tecnécio / Marcação por Isótopo / Anticorpos Idioma: Es Revista: Rev Esp Med Nucl Assunto da revista: MEDICINA NUCLEAR Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fragmentos de Imunoglobulinas / Tecnécio / Marcação por Isótopo / Anticorpos Idioma: Es Revista: Rev Esp Med Nucl Assunto da revista: MEDICINA NUCLEAR Ano de publicação: 1998 Tipo de documento: Article País de afiliação: Cuba País de publicação: Espanha