A P-type ATPase from the aquatic fungus Blastocladiella emersonii similar to animal Na,K-ATPases.
Biochim Biophys Acta
; 1383(2): 183-7, 1998 Apr 02.
Article
em En
| MEDLINE
| ID: mdl-9602120
We have cloned a P-type ATPase gene from the aquatic fungus Blastocladiella emersonii (BePAT1) using a probe obtained with degenerate oligonucleotides, corresponding to two amino acid sequences highly conserved among all P-type ATPase isoforms, and the polymerase chain reaction technique. Nucleotide sequence analysis revealed a 3.4 kb open reading frame encoding a putative peptide of 1080 amino acid residues with a calculated molecular mass of 119 kDa, which presents all diagnostic features of P-type transporting ATPases. Comparison to other members of the family and phylogenetic analyses have shown that the BePAT1 protein belongs to the subfamily of Na,K- and H,K-ATPases, indicating that the divergence between the alpha-subunit of the Na,K-ATPase and other members of the P-type ATPase family has occurred before the divergence between the animal and fungal lineages in evolution.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocladiella
/
Adenosina Trifosfatases
/
ATPase Trocadora de Sódio-Potássio
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Holanda