Entamoeba histolytica: collagen-induced AP-1 DNA binding activity.
FEMS Microbiol Lett
; 159(2): 187-92, 1998 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-9580078
The interaction of Entamoeba histolytica trophozoites with collagen induces the synthesis and release of electron-dense granules containing a collagenase activity that is an important factor in the pathogenicity of the parasite. The binding is thought to be mediated by an 'integrin-like' collagen receptor. In the signal transduction mechanisms activated by collagen, pp125FAK and p42MAPK are involved. Using immunoprecipitation assays coupled to Western blot analysis, we demonstrate here the collagen-dependent association of paxillin and Src with pp125FAK. Furthermore, collagen induces a time-dependent increase in the DNA binding activity of the activator protein 1, which is well correlated with an increase in Fos expression. Our results suggest that a stimulus-transcription coupling triggered by collagen in E. histolytica trophozoites might activate or repress genes involved in tissue invasiveness.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Colágeno
/
Fator de Transcrição AP-1
/
Entamoeba histolytica
Limite:
Animals
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Reino Unido