Separation and mapping of chromosomes of parasitic protozoa.
Mem Inst Oswaldo Cruz
; 92(6): 815-9, 1997.
Article
em En
| MEDLINE
| ID: mdl-9566214
Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demonstrate that the application of two dimensional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachromosomal genome organization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento Cromossômico
/
Eucariotos
Limite:
Animals
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França
País de publicação:
Brasil