Rotavirus G and P types circulating in Brazil: characterization by RT-PCR, probe hybridization, and sequence analysis.
Arch Virol
; 141(12): 2365-74, 1996.
Article
em En
| MEDLINE
| ID: mdl-9526543
ABSTRACT
PIP: Rotavirus is an important cause of acute gastroenteritis in many species of animals and humans, and is responsible for about 870,000 deaths annually among children under 5 years old, mostly in developing countries. Rotavirus disease will most likely be controlled through the development and widespread use of an effective vaccine. In anticipation of the possible use of such a vaccine in Brazil, the authors characterized the most common strains of rotavirus in circulation in the country. Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the P and G genotypes of 130 culture-adapted rotavirus strains isolated from 181 fecal specimens of children under age 5 years from 9 states and the Federal District of Brazil. The 4 genotypes found most commonly worldwide were also common in Brazil, with P[8]G1 being the most prevalent in 43% of cases, followed by P[4]G2 (12%), P[8]G3 (6%), and P[8]G4 (6%). The unusual types P[8]G5, P[6]G2, P[9]G1, and P[9]G3 were responsible for 12% of the cases, and mixed infections were responsible for 21% of cases. Genotype G5 strains were detected in specimens collected in all 9 areas surveyed from all 4 regions of Brazil. This unusual strain diversity in Brazil suggests that when tetravalent rotavirus vaccines currently being developed are introduced into Brazil, laboratory surveillance will be key to monitoring protection against unusual strains, especially those of genotype 5, as well as new reassortants which may evolve from the pool of children with mixed infections.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Rotavirus
/
Rotavirus
/
Proteínas do Capsídeo
/
Antígenos Virais
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Child, preschool
/
Humans
/
Infant
/
Newborn
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Áustria