[The action of epidermal growth factor on the development of cultured striatum cells]. / Acción del factor de crecimiento epidérmico sobre el desarrollo de las células estriatales en cultivo.
Rev Neurol
; 25(146): 1511-7, 1997 Oct.
Article
em Es
| MEDLINE
| ID: mdl-9462969
INTRODUCTION: Epidermic growth factor (EGF) has a neurotrophic mitogenic effect on different cell populations in the nervous system. This is modulated by the stage of development and microenvironment of the cells. OBJECTIVE AND METHODS: In this paper we describe the action of EGF on embryonic striatum cells of a culture system dissociated from neurons and glias. The cell culture is prepared from 16-17 day rat embryos. In the system used, the cell population was cultured for 20-24 hours in a medium containing serum. This medium was later replaced by a mixture of specific nutrients and treated for 6 days with 20 mg/ml of EGF. RESULTS AND CONCLUSIONS: The substitution of serum during the initial period of development led to an appreciable reduction in the living cells in the treated cultures and in the controls. The surviving cells were mainly cellular precursors, taking into account their morphological characteristics and capacity for proliferation. The effect of EGF was seen in an increase in the number of cells and was shown to be a stimulus to the proliferation of neuronal and astrocyte precursors. The specific activity of choline acetyl-transferases determined in the cultures at 16 days showed differentiation of a cholinergic neurons subpopulation, which responded to treatment with nerve growth factor with an increase in the activity of this enzyme.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Substâncias de Crescimento
/
Corpo Estriado
/
Fator de Crescimento Epidérmico
Limite:
Animals
Idioma:
Es
Revista:
Rev Neurol
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Espanha