Polyspermy in Bufo arenarum oocytes matured in vitro.
Zygote
; 5(3): 267-71, 1997 Aug.
Article
em En
| MEDLINE
| ID: mdl-9460912
Full-grown ovarian oocytes of the amphibian Bufo arenarum were induced to mature in vitro by removing the follicular layers (spontaneous maturation) or by treatment with progesterone (hormone-induced maturation). These oocytes were then treated with trypsin and inseminated with homologous spermatozoa. Oocytes matured in vivo that had not undergone any influence of the oviducts (coelomic oocytes), inseminated under the same experimental conditions, were used as controls. The results show that oocytes induced to mature in vitro and exhibiting apparently normal signs of activation were polyspermic. In fact, 2 h after insemination numerous functioning pronuclei could be observed in the animal hemisphere. These results suggest that even though the oocytes which matured in vitro were able to undergo activation after insemination, they were unable to establish an effective block to polyspermy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Interações Espermatozoide-Óvulo
/
Bufo arenarum
/
Fertilização in vitro
Limite:
Animals
Idioma:
En
Revista:
Zygote
Assunto da revista:
EMBRIOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido