Biochemistry of the evolution of Triatoma infestans. XII. Biosynthesis and secretion of a very high density lipoprotein.
Acta Physiol Pharmacol Ther Latinoam
; 47(2): 77-86, 1997.
Article
em En
| MEDLINE
| ID: mdl-9339237
Biosynthetic processes related to the production of an insect hexamerin, very high density lipoprotein (VHDL), have been examined in the fat body of fifth-instar nymph and adult Triatoma infestans. Fat bodies were incubated in vitro with [3H]leucine and the incubation media were precipitated using a specific antiserum. The SDS-polyacrylamide gel electrophoresis followed by blotting on nitrocellulose showed that both larval and adult fat body secreted the VHDL subunit. Moreover, the radiolabel recovered in this subunit is indicative of the de novo synthesis. When the incubation medium was subjected to density gradient ultracentrifugation, a radiolabeled fraction was found at density 1.27 g/ml, value identical to the hemolymph circulating VHDL, indicating that the secreted apoprotein is combined with lipids. The SDS-polyacrylamide gel electrophoresis and immunoblotting of this fraction corroborated the presence of the VHDL-apoprotein. These results demonstrate that the fat body of T. infestans is able to synthesize the protein subunit which is associated to lipids as a lipoprotein particle that is released into the medium as VHDL.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Triatoma
/
Proteínas de Insetos
/
Lipoproteínas HDL
Limite:
Animals
Idioma:
En
Revista:
Acta Physiol Pharmacol Ther Latinoam
Assunto da revista:
FARMACOLOGIA
/
FISIOLOGIA
/
TERAPEUTICA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Argentina