Influence of bile on beta-galactosidase activity and cell viability of Lactobacillus reuteri when subjected to freeze-drying.

de Valdez, G F; Martos, G; Taranto, M P; Lorca, G L; Oliver, G; de Ruiz Holgado, A P

de Valdez, G F; Martos, G; Taranto, M P; Lorca, G L; Oliver, G; de Ruiz Holgado, A P.

Afiliação

de Valdez GF; Centro de Referencia para Lactobacilos (CERELA), Tucumán, Argentina.

J Dairy Sci ; 80(9): 1955-8, 1997 Sep.

Article em En | MEDLINE | ID: mdl-9313134

RESUMO

The effect of bile on beta-galactosidase activity and cell viability was investigated using two strains of Lactobacillus reuteri that were subjected to freeze-drying. In the presence of 0.15% oxgall, beta-galactosidase activity of the whole cells was significantly increased. After lyophilization, the cultures that had been treated with oxgall showed a low survival rate without changes in beta-galactosidase activity. The poor resistance of the cells to damage from freeze-drying might be related to the presence of membranous structures containing simple folds and buds of the cell membrane, as was observed by transmission electron microscopy.

Assuntos

Bile/fisiologia; Liofilização; Lactobacillus/enzimologia; Lactobacillus/fisiologia; beta-Galactosidase/metabolismo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bile / Beta-Galactosidase / Liofilização / Lactobacillus Idioma: En Revista: J Dairy Sci Ano de publicação: 1997 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos

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