Evidence for hybridization by multilocus enzyme electrophoresis and random amplified polymorphic DNA between Leishmania braziliensis and Leishmania panamensis/guyanensis in Ecuador.
J Eukaryot Microbiol
; 44(5): 408-11, 1997.
Article
em En
| MEDLINE
| ID: mdl-9304809
The taxonomic attribution of four Leishmania stocks isolated from humans in Ecuador has been explored by both multilocus enzyme electrophoresis and random amplified polymorphic DNA. For three loci, MLEE results showed patterns suggesting a heterozygous state for a diploid organism, while the corresponding homozygous states are characteristic of the Leishmania panamensis/guyanensis complex and Leishmania braziliensis, respectively. Other enzyme loci showed characters attributable to either the L. panamensis/ guyanensis complex or L. braziliensis. RAPD profiles exhibited for several primers a combination of the Leishmania panamensis/ guyanensis complex and L. braziliensis characters. These data hence suggest that the four stocks are the result of hybridization between L. panamensis/guyanensis and L. braziliensis. MLEE data show that the results cannot be attributed to either mixture of stocks, or an F1 in the framework of a simple Mendelian inheritance.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Leishmania braziliensis
/
Quimera
/
Leishmania guyanensis
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Humans
País/Região como assunto:
America central
/
America do sul
/
Brasil
/
Ecuador
/
Panama
Idioma:
En
Revista:
J Eukaryot Microbiol
Assunto da revista:
MICROBIOLOGIA
/
PARASITOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
França
País de publicação:
Estados Unidos