[Diagnosis of human neurocysticercosis by ELISA-IgG using a purified antigen]. / Diagnóstico de la neurocisticercosis humana mediante ELISA-IgG usando un antígeno purificado.
Bol Chil Parasitol
; 51(3-4): 85-90, 1996.
Article
em Es
| MEDLINE
| ID: mdl-9302781
An analysis of immunodiagnosis data for human neurocysticercosis (NC) by ELISA-IgG, complement fixation test (CFT) and indirect hemagglutination test (IHAT) in 67 serum samples and 54 cerebrospinal fluids (CSF) from confirmed cases of different hospitals from the Metropolitan Area of Santiago, Chile, was performed. The cut-off value was determined by using serum samples from 60 apparently healthy persons, whose absorbance values were the mean plus three standard deviations. The sensitivity of ELISA was 97.0% and 100% for serum samples and CSF respectively. This assay was considered of statistical significance (p < 0.05) when it was compared with CFT. Specificity was established by testing a purified antigen over 109 different helminthiasis serum samples, 185 neurological affections other than NC and 60 control samples. A 98.3% of global specificity was found. The use of ELISA-IgG and a purified antigen in the approach of immune diagnosis of NC is considered a useful assay, particularly if it is performed on paired serum/CSF samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina G
/
Cisticercose
/
Ensaio de Imunoadsorção Enzimática
/
Doenças do Sistema Nervoso Central
/
Cysticercus
/
Antígenos de Helmintos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Humans
Idioma:
Es
Revista:
Bol Chil Parasitol
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Chile