Oxidative stress and in vivo chemiluminescence in mouse skin exposed to UVA radiation.
J Photochem Photobiol B
; 38(2-3): 215-9, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9203384
Mouse skin was exposed to UVA radiation (320-400 nm). The in vivo chemiluminescence of the skin was measured after irradiation. Chemiluminescence showed a maximum 13-fold increase (control emission, 10 +/- 1 cps cm-2) after 45-60 min of exposure to UVA, with no further increase with 60 min additional exposure. Spectral analysis of the emitted chemiluminescence showed that the principal species emitted in the 400-500 nm range. Topical application with alpha-tocopherol (10% v/w) and beta-carotene (1 mM) greatly reduced the UVA-induced skin chemiluminescence. Thiobarbituric acid reactive substance (TBARS) levels were increased by 130% in skin homogenates after 2 h of exposure to UVA (control value, 77 +/- 14 nmol malonaldehyde equivalents (g tissue)-1). The activities of antioxidant enzymes in skin homogenates were decreased after 2 h of irradiation: the superoxide dismutase (SOD) activity (control value, 181 +/- 10 U SOD (g tissue)-1) was decreased by 40% and the catalase activity (control value, 1.34 +/- 0.14 pmol (g tissue)-1) was decreased by 45%. In vivo chemiluminescence appears to be a suitable method for following the kinetics of the oxidative stress processes and for testing the effect of topical application with antioxidant and photoprotective agents.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Raios Ultravioleta
/
Estresse Oxidativo
Limite:
Animals
Idioma:
En
Revista:
J Photochem Photobiol B
Assunto da revista:
BIOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Suíça