Human placental ecto-enzymes: studies on the plasma membrane anchorage and effect of inhibitors of ATP-metabolizing enzymes.
Res Commun Mol Pathol Pharmacol
; 96(1): 14-24, 1997 Apr.
Article
em En
| MEDLINE
| ID: mdl-9178364
The human placental microvillar membrane contains several ectoenzymes, including 5'-nucleotidase, alkaline phosphatase and ATP-diphosphohydrolase (ATP-DPH), which might be involved in the extracellular metabolism of nucleotides. The type of anchorage to the plasma membrane of the two first enzymes has been shown to be via a glycosyl-phosphatidylinositol. In the present study, using an enzymatic approach, we show that the ATP-DPH should be attached to the plasma membrane through a different type of anchorage. We were also interested in the search of compounds which could interact differentially with this enzyme to be used as a tool for studying the other two hydrolytic enzymes in the presence of ATP-DPH. Here we report several inhibitors of ecto-ATPases which seem to be a useful tool for studying these three enzymes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Apirase
/
Placenta
/
5'-Nucleotidase
/
Fosfatase Alcalina
/
Inibidores Enzimáticos
Limite:
Female
/
Humans
Idioma:
En
Revista:
Res Commun Mol Pathol Pharmacol
Assunto da revista:
BIOLOGIA MOLECULAR
/
FARMACOLOGIA
/
PATOLOGIA
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Estados Unidos