Dried blood spots collected on filter paper: an international resource for the diagnosis and genetic characterization of human immunodeficiency virus type-1.
Mem Inst Oswaldo Cruz
; 91(3): 351-8, 1996.
Article
em En
| MEDLINE
| ID: mdl-9040855
The collection of dried blood spots (DBS) on filter paper provides a powerful approach for the development of large-scale, population-based screening programs. DBS methods are particularly valuable in developing countries and isolated rural regions where resources are limited. Large numbers of field specimens can be economically collected and shipped to centralized reference laboratories for genetic and (or) serological analysis. Alternatively, the dried blood can be stored and used as an archival resource to rapidly establish the frequency and distribution of newly recognized mutations, confirm patient identity or track the origins and emergence of newly identified pathogens. In this report, we describe how PCR-based technologies are beginning to interface with international screening programmes for the diagnosis and genetic characterization of human immunodeficiency virus type 1 (HIV-1). In particular, we review recent progress using DBS specimens to resolve the HIV-1 infection status of neonates, monitor the genetic evolution of HIV-1 during early infancy and establish a sentinel surveillance system for the systematic monitoring of HIV-1 genetic variation in Asia.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papel
/
Sorodiagnóstico da AIDS
/
Coleta de Amostras Sanguíneas
/
Síndrome da Imunodeficiência Adquirida
/
HIV-1
/
Filtração
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
/
Infant
/
Newborn
Idioma:
En
Revista:
Mem Inst Oswaldo Cruz
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Canadá
País de publicação:
Brasil