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An ultrastructural and cytochemical study of Tritrichomonas foetus-eosinophil interaction.
de Azevedo, N L; de Souza, W.
Afiliação
  • de Azevedo NL; Institute of Biophysics Carlos Chagas Filho, Federal University of Rio de Janeiro, Brazil.
J Submicrosc Cytol Pathol ; 28(2): 243-9, 1996 Apr.
Article em En | MEDLINE | ID: mdl-8964048
The fine structure of the process of interaction between uncoated and antibody-coated Tritrichomonas foetus with rat peritoneal eosinophils was studied. Eosinophils were purified by discontinuous Metrizamide gradient. Agglutination and immunofluorescence microscopy showed that T.foetus grown in a medium supplemented with different kinds of serum, antibodies present in it opsonize the parasites. Parasites incubated in the presence of anti-T.foetus rabbit serum attach to and are ingested by eosinophils. No such attachment was observed with trypsin-treated parasites. Attachment of antibody-coated parasites to the eosinophils induced their degranulation with the release of granule contents onto parasite surface causing its destruction. Hyperimmune serum was shown to be required for both attachment and ingestion of the parasites. The cytochemical localization of basic proteins and peroxidase showed that leucocyte granules fused with parasite-containing phagocytic vacuoles. Images were obtained suggesting that the binding of the parasites to the eosinophil surface triggers an exocytic process with release of the granule content onto the parasite surface.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tritrichomonas foetus / Eosinófilos Limite: Animals Idioma: En Revista: J Submicrosc Cytol Pathol Assunto da revista: PATOLOGIA Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Brasil País de publicação: Itália
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tritrichomonas foetus / Eosinófilos Limite: Animals Idioma: En Revista: J Submicrosc Cytol Pathol Assunto da revista: PATOLOGIA Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Brasil País de publicação: Itália