Distribution of HIV-1 subtypes seen in an AIDS clinic in Sao Paulo City, Brazil.
AIDS
; 10(13): 1579-84, 1996 Nov.
Article
em En
| MEDLINE
| ID: mdl-8931795
ABSTRACT
PIP: Serum samples from 80 consecutive HIV-1-infected individuals presenting to the Immunodeficiency Clinic at the University of Sao Paulo in 1993 were analyzed to determine the distribution of HIV-1 subtypes in the city. Peripheral blood mononuclear cells (PBMC) were separated using Ficoll-Hypaque gradient, a portion was used for routine CD4 counts, and the rest were frozen. PBMC were proteinase-K-digested and DNA-purified by organic extraction. The samples were amplified for the env region of HIV, and envelope sequence subtypes determined by heteroduplex mobility analysis using prototypic subtypes as references. A subset was also sequenced through the C2-V3 region of env. 69 samples yielded env polymerase chain reaction product enabling subtype determination. The samples which did not amplify had low DNA yields. Among 12 IV-drug users or their sex partners, four were typed as clade F and eight as clade B. 43 homosexual men or female sex partners of bisexual men were typed as clade B. The 14 additional cases with no known risk factor were typed as clade B.
Palavras-chave
Americas; Brazil; Demographic Factors; Developing Countries; Diseases; Examinations And Diagnoses; Hiv; Hiv Infections; Laboratory Examinations And Diagnoses; Laboratory Procedures; Latin America; Population; Population Characteristics; Research Report; South America; Urban Population; Viral Diseases
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Genes env
/
Síndrome da Imunodeficiência Adquirida
/
HIV-1
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Female
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
AIDS
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Reino Unido