Use of the in vitro induced antibody production test (IVIAP) to elucidate inconclusive status of HIV-1 infection.
Diagn Microbiol Infect Dis
; 25(2): 65-9, 1996 Jun.
Article
em En
| MEDLINE
| ID: mdl-8882891
Studies have demonstrated the diagnostic usefulness of in vitro production of virus-specific antibodies by peripheral blood mononuclear cells (PMBC) from HIV-infected subjects. We evaluated the IVIAP (in vitro induced antibody production) by lympocytes from peripheral blood of 30 adult patients. Samples included 10 patients with inconclusive status of HIV-1 infection on previous serologic screening (2 blood donors, 8 with high risk behavior), 10 AIDS patients (CDC II-IV), and 10 known seronegative subjects. Positive IVIAP results were obtained on 2 of 10 inconclusive cases, earlier than seroconversion would demonstrate. The 8 remaining cases resulted in a negative outcome on IVIAP test, even in 2 seronegative homosexuals presenting Kaposi's sarcoma. Results showed 100% concordance either with a clinical/serological follow-up done after IVIAP tests or PCR performed for Kaposi's cases. Also, no false-positive or false-negative was detected on control groups. We concluded that IVIAP could identify the real status of HIV infection in a shorter time and with low cost compared to conventional serological methods currently available. The IVIAP test is easily performed so that can be considered as a routine supplemental test.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-HIV
/
Síndrome da Imunodeficiência Adquirida
/
HIV-1
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos