Glucose-6-phosphate dehydrogenase activity in erythrocytes from chronically copper-poisoned sheep.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol
; 114(3): 197-200, 1996 Jul.
Article
em En
| MEDLINE
| ID: mdl-8856959
The present study was undertaken to investigate the capacity to generate reducing equivalents in erythrocytes from experimentally copper-poisoned sheep. Ten ewes were dosed orally with CuSO4 to induce the Cu toxicity. Copper dosing was stopped at the first day of hemolysis. The activity of glucose-6-phosphate dehydrogenase (G6PD) in the erythrocytes, the levels of reduced glutathione (GSH) and glucose (in serum and erythrocytes) was examined at frequent intervals. The copper-poisoned sheep had reduced levels (25-35% less) of glucose in serum and erythrocytes than controls. The activity of G6PD in erythrocytes from sheep was 50-60% of typical levels found in human erythrocytes. Immediately before the hemolytic period, the copper-poisoned sheep showed decreased activity of G6PD, declining to 65% of the initial activity. In addition, we found decreased blood levels of reduced GSH in copper-poisoned sheep. There appears to be a relationship between decreased capacity to generate reducing equivalents and the overload of copper in sheep erythrocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cobre
/
Eritrócitos
/
Glucosefosfato Desidrogenase
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol
Assunto da revista:
ENDOCRINOLOGIA
/
FARMACOLOGIA
/
TOXICOLOGIA
Ano de publicação:
1996
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos