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[Genotoxicity resulting from a complex mixture of environmental origin using 2 bacterial systems]. / Estudio de la genotoxicidad resultante de una mezcla compleja de origen ambiental mediante dos sistemas bacterianos.
López, L; Moretton, J.
Afiliação
  • López L; Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol ; 28(1): 1-8, 1996.
Article em Es | MEDLINE | ID: mdl-8815455
The genotoxicity of environmental complex mixtures was tested with Ames mutagenicity assay and with Bacillus subtilis rec assay for estimation of primary DNA damage. Soil samples obtained from an area contaminated with petrochemical wastes were sequentially extracted with ethyl ether and methanol. After evaporation to dryness the extracts were resuspended in dimethylsulfoxide for their use in biological tests. No mutagenic response was detected with the Ames test. Ether extract from parcel 1 showed an unusually high toxicity for Salmonella typhimurium TA100 and induced DNA damage in Bacillus subtilis with a clear relationship dose-genotoxic response. The analysis of the results obtained in both assays and the nature of the samples indicate that the genotoxicity detected in the complex mixture is compatible with the one caused by DNA intercalating compounds.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Poluentes do Solo / Bacillus subtilis / DNA Bacteriano / Resíduos Industriais / Substâncias Intercalantes / Testes de Mutagenicidade Idioma: Es Revista: Rev Argent Microbiol Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella typhimurium / Poluentes do Solo / Bacillus subtilis / DNA Bacteriano / Resíduos Industriais / Substâncias Intercalantes / Testes de Mutagenicidade Idioma: Es Revista: Rev Argent Microbiol Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina