Your browser doesn't support javascript.
loading
Entamoeba histolytica: fecal antigen capture immunoassay for the diagnosis of enteric amebiasis by a monoclonal antibody.
Urdaneta, H; Rangel, A; Martins, M S; Muñoz, J F; Hernández, M.
Afiliação
  • Urdaneta H; Institute of Clinical Immunology, Los Andes University, Venezuela. uhaydee@ing.ula.ve
Rev Inst Med Trop Sao Paulo ; 38(1): 39-44, 1996.
Article em En | MEDLINE | ID: mdl-8762638
Amebiasis continues to be of epidemiological importance in underdeveloped countries. Clinical diagnosis and epidemiological setting in a region are based on the fecal microscopic identification of cysts or trophozoites. This procedure requires well trained personnel, is laborious, of low sensitivity and frequently yields false-positives results. The present study was designed to develop an immunoenzymatic fecal 96 kDa antigen capture test (COPROELISA-Eh) more sensitive and specific than microscopic diagnosis of amebiasis. Triplicates of 177 stool samples processed by the formol-ether concentration method, were defined as positive or negative by three experienced microscopic observers. Another aliquot was submitted to the antigen capture test by a monoclonal antibody against a specific membrane antigen of pathogenic strains of Entamoeba histolytica. Optical densities were interpreted as positive when they exceeded the mean value of negative samples plus two standard deviations. COPROELISA-Eh showed a 94.4% sensitivity, 98.3% specificity, 96.2% positive predictive value and 97.6% negative predictive value for the detection of E. histolytica in feces. COPROELISA-Eh is more sensitive and specific than microscopic examination, does not require specially trained personnel and allows the simultaneous processing of a large number of samples.
Assuntos
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Entamoeba histolytica / Entamebíase Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Brasil
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Entamoeba histolytica / Entamebíase Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals / Humans Idioma: En Revista: Rev Inst Med Trop Sao Paulo Ano de publicação: 1996 Tipo de documento: Article País de afiliação: Venezuela País de publicação: Brasil