Foreign anion substitution for chloride in neonatal human red cells. Effect on cellular volume.
Arch Physiol Biochem
; 103(1): 91-7, 1995 Apr.
Article
em En
| MEDLINE
| ID: mdl-8574784
The effect of replacement of chloride by thiocyanate has been investigated in neonatal human red cells. On incubating these cells in isotonic SCN- medium, a rapid cellular swelling was observed, which was not evident in Cl- media. Incubation in hypertonic SCN- medium showed a rapid osmotic shrinkage followed by reswelling back to the initial volume. This regulatory volume increase was completed in a shorter time than in Cl- medium. Whole water accumulated by the cells in both experimental conditions can be accounted for by net Na+ uptake (twofold augmentation with respect to initial value). Amiloride inhibits specifically both cellular swelling and the Na+ content increase in these experimental conditions. Similar experiments in neonatal red cells incubated in NO3- media, as well as in adult red cells placed in NO3- or SCN- media, did not exhibit the same response. The findings suggest that the response of neonatal red cells in a SCN- containing media was mediated by activation of a Na+/H+ antiport mechanism.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tiocianatos
/
Cloretos
/
Eritrócitos
Limite:
Adult
/
Humans
/
Newborn
Idioma:
En
Revista:
Arch Physiol Biochem
Assunto da revista:
BIOQUIMICA
/
FISIOLOGIA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Reino Unido