The histidine operon of Azospirillum brasilense: organization, nucleotide sequence and functional analysis.
Res Microbiol
; 144(3): 187-200, 1993.
Article
em En
| MEDLINE
| ID: mdl-8210676
A 3457-base pair fragment of Azospirillum brasilense DNA which complemented mutations in the hisA and hisF genes of Escherichia coli was sequenced. The sequence analysis revealed the presence of six major contiguous open reading frames (ORF). The comparison of the predicted amino acid sequence of these ORF with those encoded by the eubacterial, archaebacterial and eukaryotic his genes sequenced thus far revealed that four of them have a significant degree of homology with the E. coli hisH, hisA, hisF and the C-terminal domain of the hisI gene products. S1 mapping experiments indicated that the putative transcription start site coincided with the AUG translational initiation codon of the hisBd gene, the first gene of the A. brasilense his operon. Downstream from the last ORF, a sequence was identified which functions as a Rho-independent transcription terminator. Comparison of amino acid sequences, gene order and organization and evolutionary aspects of the A. brasilense his cluster are discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óperon
/
Azospirillum brasilense
/
Histidina
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Res Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Itália
País de publicação:
França