Myenteric neurons of the mouse small intestine. Morphometry and acetylcholinesterase activity.
Braz J Med Biol Res
; 27(1): 101-8, 1994 Jan.
Article
em En
| MEDLINE
| ID: mdl-8173525
1. The myenteric plexus of the small intestine of five C57BL/6J male 5-month-old mice was investigated in whole-mount preparations of the muscularis externa by Giemsa staining and by the acetylcholinesterase (AChE) histochemical technique. 2. The neuronal density was 20212 +/- 3038/cm2 (mean +/- SEM) in the duodenum, 21948 +/- 1488/cm2 in the jejunum, and 25048 +/- 2356/cm2 in the ileum. The difference in neuronal density between duodenum and ileum was statistically significant (P < 0.05). The total serosal surface area of the small intestine was about 30.80 +/- 2.90 cm2, and the total number of neurons was estimated at about 690,000. 3. The neuronal cell and nucleus profile areas ranged, respectively, from 23 to 325 microns 2 and from 6 to 95 microns 2 in the small intestine of the mice studied. There were no significant differences in any of the 3 regions in terms of average neuronal cell or nucleus profile areas. 4. For the histochemical demonstration of AChE, the "direct coloring" copper ferrocyanide method was used. AChE-positive nerve fibers were distributed in the myenteric plexus which was formed by a primary meshwork of relatively large nerve bundles and a secondary meshwork of finer nerve bundles. Most of the neurons of the plexus displayed AChE activity in the cytoplasm though the neurons presented different reaction intensities.(ABSTRACT TRUNCATED AT 250 WORDS)
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Acetilcolinesterase
/
Intestino Delgado
/
Plexo Mientérico
Limite:
Animals
Idioma:
En
Revista:
Braz J Med Biol Res
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil