Characterization and properties of steroid binding protein in Bufo arenarum serum.
Mol Reprod Dev
; 38(4): 364-72, 1994 Aug.
Article
em En
| MEDLINE
| ID: mdl-7980944
Serum steroid binding properties of mature Bufo arenarum females were studied. Binding data obtained using charcoal adsorption assay and equilibrium dialysis methods indicates a single protein, named Bufo arenarum sex binding protein (Ba SBP), which binds 5 alpha-dihydrotestosterone (DHT), testosterone (T), and estradiol-17 beta (E2) with high affinity (10(-7) M-1 - 10(8) M-1) and fair capacity (10(-6) M). Scatchard plot analysis demonstrated the coexistence of two binding sites. Ba SBP has a sedimentation coefficient of 5.2 S in sucrose gradient centrifugation in low salt and under steady-state conditions. The specificity of this protein, determined by competitive binding experiments, is comparable to human SBP. DHT and T bind with higher affinity than E2. Estriol and estrone competed poorly, while diethylstilbestrol and C21 steroids did not compete. The binding capacity of this protein is under estrogenic control.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bufo arenarum
/
Globulina de Ligação a Hormônio Sexual
Limite:
Animals
Idioma:
En
Revista:
Mol Reprod Dev
Assunto da revista:
BIOLOGIA MOLECULAR
/
MEDICINA REPRODUTIVA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos