Amphibian cross-fertilization and polyspermy.
J Exp Zool
; 269(6): 560-5, 1994 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-7931127
A string of oocytes from Bufo arenarum (Ba) was inseminated with spermatozoa from Ba, Leptodactylus chaquensis (Lch), or Bufo paracnemis (Bp). Homologous insemination resulted in monospermic eggs and normal development, while in the case of heterologous fertilization both polyspermy and abnormal development were the rule. Oocytes from Ba inseminated with homologous spermatozoa, when reinseminated 30 sec after the first insemination with heterologous spermatozoa, exhibit a high rate of polyspermy and abnormal development. A lower rate of abnormalities was observed when reinsemination was carried out 60 sec after the first insemination. Normal development and an absence of polyspermy were observed in the case of eggs reinseminated 300 sec after initial insemination. This result indicated that the electrophysiological blockage of fertilization either was not triggered or was ineffective. The establishment of the permanent blockage of polyspermy, either by artificial activation of oocytes or as a result of their incubation with the products of the cortical granules, inhibited penetration of Ba eggs by heterologous sperm.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
/
Fertilização
/
Hibridização Genética
Limite:
Animals
Idioma:
En
Revista:
J Exp Zool
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos