Perspectives and limitations of the mycobacterial expression system.
Arch Med Res
; 25(4): 451-3, 1994.
Article
em En
| MEDLINE
| ID: mdl-7858406
To develop multivalent vaccines expressing foreign antigens in BCG strains, a genetic system for BCG and Mycobacterium smegmatis is being developed that uses vectors derived from the M. fortuitum pAL5000 plasmid. In this paper we present recent advances of our laboratory in this line of research: i) optimal conditions for M. fortuitum electroporation, ii) demonstration that host range properties of pAL5000-based vectors depend on certain open reading frames, iii) cloning of expression signals derived from the BCG Moreaux 65 kD antigen, and iv) construction of expression vectors for M. smegmatis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Clonagem Molecular
/
Mycobacterium
Idioma:
En
Revista:
Arch Med Res
Assunto da revista:
MEDICINA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Cuba
País de publicação:
Estados Unidos