[An evaluation of the direct agglutination test for the diagnosis of "mal de caderas" in horses]. / Evaluación de la prueba de aglutinación directa en el diagnostico del mal de caderas en equinos.
Rev Inst Med Trop Sao Paulo
; 36(3): 211-5, 1994.
Article
em Es
| MEDLINE
| ID: mdl-7855484
The usefulness of the direct agglutination test (DA) to diagnose Mal de Caderas disease was evaluated. Forty four sera samples from two lots of horses with natural T. evansi infection (Lot 1 and Lot 2) were used. Thirteen (81.2%) of sixteen horses in which parasites were isolated gave positive agglutination titres (> or = 1:512) in the DA test. Treatment of these positive sera with 2-mercaptoethanol drops three to eight dilutions the agglutination titres in twelve samples (92%), showing the IgM nature of these antibodies. The DA test was also positive in seventeen of twenty eight horses in which parasites could not be detected. Five T. evansi infected horses, Lot three, which had high antibodies levels in the DA test, were treated with Naganol (Bayer-Germany). In four animals these antibodies were mainly IgM. In agreement with negative control for parasites, two months after treatment, IgM could not be detected while IgG antibodies remained detectable in low titres 12 months in three of the five horses. Fifty control horses sera from a T. evansi free area were AD negative. The DA and DA+2-ME are recommended as a routine method to diagnose Mal de Caderas disease in combination with parasitological diagnostic methods.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tripanossomíase
/
Testes de Aglutinação
/
Doenças dos Cavalos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Es
Revista:
Rev Inst Med Trop Sao Paulo
Ano de publicação:
1994
Tipo de documento:
Article
País de publicação:
Brasil