Effect of sodium dodecyl sulfate on lipase of Candida lipolytica.
Appl Biochem Biotechnol
; 49(2): 93-9, 1994 Nov.
Article
em En
| MEDLINE
| ID: mdl-7818267
The effects of sodium dodecyl sulfate on extracellular lipase produced by Candida lipolytica have been studied. The microorganism was grown in culture medium containing different sodium dodecyl sulfate concentrations added to the culture at different intervals of growth. The extracellular lipase activity was not detected when the treated culture supernatants were directly tested in Yeast Mold Agar-Triolein-Rhodamine plates, regardless of surfactant addition time and concentrations. However, after ammonium sulfate precipitation and dialysis, the extracellular lipase activity could be recovered. Therefore, the surfactant, under the experimental conditions used here, does not seem to be able to inhibit lipase production, but it does inhibit the enzyme activity because of its presence in the mixture of the reaction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dodecilsulfato de Sódio
/
Candida
/
Lipase
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Estados Unidos