Selective inhibition of cytosine-DNA methylases by polyamines.

Ruiz-Herrera, J; Ruiz-Medrano, R; Domínguez, A

Ruiz-Herrera, J; Ruiz-Medrano, R; Domínguez, A.

Afiliação

Ruiz-Herrera J; Departmento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto., Mexico.

FEBS Lett ; 357(2): 192-6, 1995 Jan 03.

Article em En | MEDLINE | ID: mdl-7805889

RESUMO

We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines. On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM. Our results indicate that the effect of polyamines on cytosine-DNA methyltransferases is rather selective and suggest a possible mode of action in vivo.

Assuntos

DNA-Citosina Metilases/antagonistas & inibidores; Poliaminas/farmacologia; DNA/metabolismo; Metilação; Espermidina/farmacologia

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poliaminas / DNA-Citosina Metilases Idioma: En Revista: FEBS Lett Ano de publicação: 1995 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

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