cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.
Biochem Cell Biol
; 72(9-10): 381-90, 1994.
Article
em En
| MEDLINE
| ID: mdl-7605609
We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenobarbital
/
Regulação Enzimológica da Expressão Gênica
/
AMP Cíclico
/
Diabetes Mellitus Experimental
/
5-Aminolevulinato Sintetase
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Biochem Cell Biol
Assunto da revista:
BIOQUIMICA
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Canadá