Membrane fluidity of Escherichia coli during heat-shock.

Mejía, R; Gómez-Eichelmann, M C; Fernández, M S

Mejía, R; Gómez-Eichelmann, M C; Fernández, M S.

Afiliação

Mejía R; Department of Biochemistry, Centro de Investigación y de Estudios Avanzados del I.P.N. México D.F., Mexico.

Biochim Biophys Acta ; 1239(2): 195-200, 1995 Nov 01.

Article em En | MEDLINE | ID: mdl-7488624

RESUMO

The excimer-forming fluorophore dipyrenylpropane has been used to measure the relative fluidity of total membranes isolated from Escherichia coli grown at 30 or 45 degrees C, or exposed to a heat-shock from 30 to 45 degrees C for various periods of time. Parallel experiments were performed using [35S]methionine pulse-labeling of cells, to study the induction of heat-shock proteins (HSPs) at different times after the sudden change in E. coli growth-temperature from 30 to 45 degrees C. Results suggest that upon an abrupt temperature upshift from 30 to 45 degrees C, membrane fluidity adjustment to the steady-state level at the high temperature, takes place during the E. coli heat-shock response.

Assuntos

Escherichia coli/fisiologia; Resposta ao Choque Térmico; Fluidez de Membrana; Corantes Fluorescentes; Proteínas de Choque Térmico/biossíntese; Indicadores e Reagentes; Pirenos; Fatores de Tempo

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Resposta ao Choque Térmico / Escherichia coli / Fluidez de Membrana Idioma: En Revista: Biochim Biophys Acta Ano de publicação: 1995 Tipo de documento: Article País de afiliação: México País de publicação: Holanda

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